Though we have not tested PneumaCult™ media for use with murine epithelial cells, you will find publications that report this application.
Examine preclinical check compounds and biologics for his or her skills to modulate the immune system.
Stream cytometry can be used to evaluate the performance of differentiation protocols With all the STEMdiff™ system, by investigating unique marker expression at Every single stage on the differentiation process. It may also be employed for other applications, like cell sorting, immunophenotyping, and purity evaluation.
Operate swiftly at this step to pool wells right into a fifteen mL tube. Notice: For a sizable-scale dissociation, use a multichannel pipette to pool cells into a sterile reagent reservoir. Include the pooled wells into a fifteen mL tube.
Carefully get rid of the supernatant with no disturbing the organoid pellet. Insert 0.five mL of desired single-cell dissociation reagent for the tube. Distinct incubation guidelines for different dissociation reagents are mentioned beneath:
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Observe: Carry on to antibody staining if performing live cell Evaluation immediately after this move. Alternatively, proceed to fixable dye staining and PFA fixation.
In the event cell clumping is noticed right after thawing cryopreserved cells, it is usually recommended to 지방이식 filter aggregated suspensions by way of a 37 µm cell strainer (e.g. Catalog #27250) for exceptional benefits. In this case, the operate time on the CellPore™ Transfection System could should be elevated to ten seconds.
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Get rid of supernatant and resuspend cells in FACS buffer. Note: It can be crucial to quench the dissociation reagent by utilizing the exact same or double the quantity in the dissociation reagent.